Steve Mahler
  1. Current research activities

    2. 1. Isolation of monoclonal antibodies against stem cell markers
    Technologies will be required to facilitate the rapid isolation of monoclonal antibodies against ES cell surface antigens and/or cytosolic proteins. The traditional technique for antibody isolation is through protein immunization followed by hybridoma production. For isolation of panels of antibodies against a potential myriad of ES cell markers, a more high throughput antibody isolation technique would be more appropriate. Immunoglobulin gene repertoire cloning combined with antibody phage display technology offer a potentially powerful tool for the isolation of specific antibodies against a purified or a complex mix of antigens. It is particularly suited to high throughput target antigen identification, protein expression profiling and functional studies.

    2. Tissue Regeneration
    I am collaborating with a group located in the School of Biomedical Engineering in the area of tissue regeneration, specifically the identification of factors involved in the regenerative process, using proteomic techniques

  2. Keywords

    3. Stem cells characterization, tissue regeneration, proteomics, monoclonal antibodies.

  3. End-user applications

    • The creation of panels of monoclonal antibodies that bind to ES cell surface antigens for various purposes; for example, characterizing differentiation pathways; affinity purification of populations of stem cells, defined by their surface markers
    • Development of proteomic techniques for the identification of stem cell marker proteins
    • Addition of growth/differentiation factors to biomaterials to aid in the tissue regenerative process

  4. Key publications

    5. I. Jespers, L.S., Roberts, A., Mahler, S.M., Winter, G. and Hoogenboom, H.R. (1994) Guiding the selection of human antibodies from phage display repertoires to a single epitope of an antigen. Bio/Technology 12, 899-903
    II. Mahler, S.M., Marquis, C.P., Brown, G., Roberts, A. and Hoogenboom, H.R. (1997) Cloning and expression of human V-genes derived from phage display libraries as fully assembled human anti-TNFa monoclonal antibodies. Immunotechnology 3, 31-43
    III. Bootcov, M.R., Bauskin, A.R., Valenzuela, S.M., Moore, A.G., Bansal, M., He, X.Y., Zhang, H.P., Donnellan, M., Mahler, S.M., Pryor, K., Walsh, B.J., Nicholson, W., Fairlie, D., Por, S.B., Robbins, J.M., and Breit, S.N. (1997) MIC-1, a novel macrophage inhibitory cytokine, .is the first member of a new family within the TGF-ß superfamily cluster. Proc. Natl. Acad. Sci. U.S.A. 94, 11514-11519
    IV. Catzel C., Lalevski H., Marquis C., Gray P.P., Van Dyke D. and Mahler S.M. Purification of recombinant human growth hormone from CHO cell culture cupernatant using preparative electrophoresis. (2003) Protein expression and purification 32, 126-134
    V. Mahler, S.M., Chin, D.Y and Van Dyk, D. (2003) The application of emerging technologies in genomics and proteomics to drug development J Pharmacy Practice and Research, 33, 7-11

  5. Outreach activities

    6. None as Yet.

  6. Key organisation membership

    7. NSW Stem Cell Network

  7. Early career researcher?

    8. No.

  8. Young investigator?

    9. No.

  9. Skills and expertise

    • Proteomics and protein purification
    • molecular biology
    • antibody engineering
    • cell culture
  10. Specialist equipment and infrastructure

    • small and large scale protein purification equipment; proteomics facilities;
    • cell culture suites; molecular biology laboratories

  11. Contact Details 

    Dr Stephen Mahler
    Address: Biotechnology and Biomolecular Sciences
    University of New South Wales, 2052, Sydney
    Country: Australia
    Phone: +61 2 9385 3899
    Fax: +61 2 9313 6710
    Email: s.mahler@unsw.edu.au

© 2004

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