Andrew Murphy
  1. Current research activities

    2. My research is focused on formulating novel ECM-growth factor complexes, in particular looking at truncated forms of vitronectin and their interactions with IGFs and IGFBPs. These ECM-growth factor complexes are been examined on HaCAT cell lines to examine their ability to stimulate cell migration and proliferation. Future studies will be focusing on making recombinant molecules of vitronectin and assessing their structural and functional revevance.

  2. Keywords

    3. ECM-growth factor complexes, wound regeneration, recombinant proteins, protein purification, structural and functional studies.

  3. End-user applications

    • Wound healing (scarless)
    • Burns
    • Ex vivo cell expansion

  4. Key publications

    5. I. Kricker, J.A., Noble., A, Hyde., C.E., Firth, S.M., Herington, A.C. & Upton, Z. (2003) Structural and functional evidence for the interaction of IGFs and IGF-binding proteins with vitronectin. Endocrinology 144, 2807-2815
    II. Blancher, C., Omri, B., Bidou, L., Pessac, B., and Crisanti, P. (1996) Nectinepsin: a new extracellular matrix protein of the pexin family. Characterization of a novel cDNA encoding a protein with an RGD cell binding motif. The Journal Biological Chemistry 271, 26220-6
    III. Pons, S., and Marti, E. (2000) Sonic hedgehog synergizes with the extracellular matrix protein vitronectin to induce spinal motor neuron differentiation. Development 127, 333-42.
    IV. Nagano, Y., Hamano, T., Nakashima, N., Ishikawa, M., Miyazaki, K., and Hayashi, M. (1992) Yolk vitronectin. Purification and differences from its blood homologue in molecular size, heparin binding, collagen binding, and bound carbohydrate. The Journal of Biological Chemistry 267, 24863-70.
    V. Zheng, X., Saunders, T. L., Camper, S. A., Samuelson, L. C., and Ginsburg, D. (1995) Vitronectin is not essential for normal mammalian development and fertility. Proceedings of the National Academy of Sciences of the United States of America 92, 12426-30.

  5. Outreach activities

    6. None as yet.

  6. Key organisation membership

    7. None as yet.

  7. Early career researcher?

    No.

  8. Young investigator?

    9. No.

  9. Skills and expertise

    • Protein purification
    • HPLC
    • Tissue culture
    • Functional assays (migration, attachment, proliferation)
    • Structural assays (binding studies)

  10. Specialist equipment and infrastructure

    • BioRad HPLC
    • ProteomeLab™ PA 800 Protein Characterisation System

© 2004

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